Eicosapentaenoic Acid Reverses the Oleic Acid-induced Reduction in <i>Per1</i> mRNA Expression in Cultured Rat Hepatocytes

Nagura, Chinami; Tanaka, Sho; Tsunemi, Akiko; Haketa, Akira; Maruyama, Takashi; Ueno, Takahiro; Fukuda, Noboru; Soma, Masayoshi; Abe, Masanori

doi:10.7150/jbm.23267

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Research Paper

Eicosapentaenoic Acid Reverses the Oleic Acid-induced Reduction in Per1 mRNA Expression in Cultured Rat Hepatocytes

Chinami Nagura¹, Sho Tanaka¹, Akiko Tsunemi¹, Akira Haketa¹, Takashi Maruyama¹, Takahiro Ueno^1✉, Noboru Fukuda¹, Masayoshi Soma², Masanori Abe¹

1. Division of Nephrology, Hypertension and Endocrinology, Department of Medicine, Nihon University School of Medicine, Tokyo, Japan;
2. Division of General Medicine, Department of Medicine, Nihon University School of Medicine, Tokyo, Japan.

Citation:

Nagura C, Tanaka S, Tsunemi A, Haketa A, Maruyama T, Ueno T, Fukuda N, Soma M, Abe M. Eicosapentaenoic Acid Reverses the Oleic Acid-induced Reduction in Per1 mRNA Expression in Cultured Rat Hepatocytes. J Biomed 2018; 3:8-12. doi:10.7150/jbm.23267. http://www.jbiomed.com/v03p0008.htm

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Abstract

The aim of the present study was to examine the conditions and chemicals that affect the expression of circadian clock-related genes in obesity. We investigated the expression of Per1 mRNA in H35 cultured rat hepatoma cells. Incubation with 100 nM dexamethasone for 2 h increased Per1 mRNA expression successfully in H35 rat hepatoma cells after 19 h or 22 h of stimulation. Further, 50 mM oleic acid (OA) loading resulted in marked accumulation of triglycerides in H35 cells and decreased the elevated levels of dexamethasone-induced Per1 mRNA after 19 h or 22 h of stimulation. Resveratrol and niacin did not show any effect on Per1 mRNA expression in H35 cells. However, eicosapentaenoic acid (EPA) reversed the OA-induced reduction in Per1 mRNA after dexamethasone stimulation in the cultured cells. These data suggest the possible role of EPA as an agent to modify the circadian clock oscillation.

Keywords: Per1, clock gene, eicosapentaenoic acid.

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APA

Nagura, C., Tanaka, S., Tsunemi, A., Haketa, A., Maruyama, T., Ueno, T., Fukuda, N., Soma, M., Abe, M. (2018). Eicosapentaenoic Acid Reverses the Oleic Acid-induced Reduction in Per1 mRNA Expression in Cultured Rat Hepatocytes. Journal of Biomedicine, 3, 8-12. https://doi.org/10.7150/jbm.23267.

ACS

Nagura, C.; Tanaka, S.; Tsunemi, A.; Haketa, A.; Maruyama, T.; Ueno, T.; Fukuda, N.; Soma, M.; Abe, M. Eicosapentaenoic Acid Reverses the Oleic Acid-induced Reduction in Per1 mRNA Expression in Cultured Rat Hepatocytes. J. Biomed 2018, 3, 8-12. DOI: 10.7150/jbm.23267.

NLM

CSE

Nagura C, Tanaka S, Tsunemi A, Haketa A, Maruyama T, Ueno T, Fukuda N, Soma M, Abe M. 2018. Eicosapentaenoic Acid Reverses the Oleic Acid-induced Reduction in Per1 mRNA Expression in Cultured Rat Hepatocytes. J Biomed. 3:8-12.

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